ID   293LAP10
AC   CVCL_DD14
SY   293Lap10
DR   Wikidata; Q54584124
RX   Patent=WO2000006751A2;
RX   PubMed=10073694;
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12586.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03023; Escherichia coli lacI (Note=Modified version LAP348 with at the N-terminal a nuclear localization signal from SV40 T antigen and at the C-terminal the transactivator domain from HSV-1 VP16/Vmw65).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 13-07-16; Last updated: 14-08-25; Version: 12
//
RX   Patent=WO2000006751A2;
RA   Graham, Frank Lawson
RA   Prevec, Ludvik Anthony
RA   Matthews, David Alan
RT   "Repressor proteins producing cell lines for the propagation of
RT   vectors.";
RL   Patent number WO2000006751A2, 10-Feb-2000.
//
RX   PubMed=10073694; DOI=10.1099/0022-1317-80-2-345;
RA   Matthews, David Alan
RA   Cummings, Derek T.
RA   Evelegh, Carole
RA   Graham, Frank Lawson
RA   Prevec, Ludvik Anthony
RT   "Development and use of a 293 cell line expressing lac repressor for
RT   the rescue of recombinant adenoviruses expressing high levels of
RT   rabies virus glycoprotein.";
RL   J. Gen. Virol. 80:345-353(1999).
//