ID   Tau RD P301S FRET Biosensor
AC   CVCL_DA04
DR   ATCC; CRL-3275
DR   Wikidata; Q54971737
RX   PubMed=25261551;
RX   PubMed=37553663;
CC   Characteristics: Transduced with 2 separate lentivirus constructs encoding the tau repeat domain (RD) with the disease-associated p.Pro301Ser (p.Pro636Ser) mutation fused to either CFP or YFP (ATCC=CRL-3275).
CC   Characteristics: Tau seeds introduced into the culture media of this cell line can nucleate the aggregation of the reporter proteins. This aggregation produces a FRET signal which can be measured via microscopy, microplate readers or flow cytometry (ATCC=CRL-3275).
CC   Transfected with: UniProtKB; Q66PV6; CFP.
CC   Transfected with: UniProtKB; P42212; YFP (modified GFP at G-65, L-68, A-72, Y-203 and L-231).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
ST   Source(s): ATCC=CRL-3275
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 12,14
ST   D16S539: 9,13
ST   D5S818: 8,9
ST   D7S820: 11
ST   TH01: 7,9.3
ST   TPOX: 11
ST   vWA: 16,19
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 13-07-16; Last updated: 02-05-24; Version: 10
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RX   PubMed=25261551; DOI=10.1073/pnas.1411649111;
RA   Holmes B.B., Furman J.L., Mahan T.E., Yamasaki T.R., Mirbaha H.,
RA   Eades W.C., Belaygorod L., Cairns N.J., Holtzman D.M., Diamond M.I.;
RT   "Proteopathic Tau seeding predicts tauopathy in vivo.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:E4376-E4385(2014).
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RX   PubMed=37553663; DOI=10.1186/s13024-023-00643-2;
RA   Lathuiliere A., Jo Y., Perbet R., Donahue C., Commins C., Quittot N.,
RA   Fan Z.-Y., Bennett R.E., Hyman B.T.;
RT   "Specific detection of Tau seeding activity in Alzheimer's disease
RT   using rationally designed biosensor cells.";
RL   Mol. Neurodegener. 18:53.1-53.13(2023).
//