ID   WAe009-A-1A
AC   CVCL_D6K3
SY   SMUDHe010-A-1A
DR   BioSamples; SAMEA114492926
DR   hPSCreg; WAe009-A-1A
DR   Wikidata; Q127384681
RX   PubMed=38507883;
CC   From: Southern Medical University; Guangzhou; China.
CC   Characteristics: Using CRISPR/Cas9 a Brainbow-2.1 R cassette that includes nuclear GFP (nGFP), EYFP, RFP (red) and membrane-bound mCerulean was introduced in the AAVS1 safe harbor locus. Stochastic recombination using incompatible lox sites can produce by inversions and excisions four different mixtures of fluorescent proteins (PubMed=38507883).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; J2JWA; mCerulean (Note=Bright cyan fluorescent protein derived from GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 02-05-24; Last updated: 10-04-25; Version: 3
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RX   PubMed=38507883; DOI=10.1016/j.scr.2024.103383;
RA   Luo Y.-Y., Zheng W., Zhong Y.-D., Liu H.-T., Yu J., Qiu B.-Y., Liu J.,
RA   Yang B.;
RT   "Generation of a human embryonic stem cell line (SMUDHe010-A-1A)
RT   carrying Brainbow cassette in the AAVS1 gene by CRISPR/Cas9-mediated
RT   homologous recombination.";
RL   Stem Cell Res. 77:103383-103383(2024).
//