ID   TBY2-41/ST
AC   CVCL_D5GR
DR   RIKEN_BRC_EPD; rpc00093
DR   Wikidata; Q127384374
RX   PubMed=28339924;
CC   Group: Plant cell line.
CC   Characteristics: Used to visualize the trans-Golgi network thanks to the integration of two fusion constructs GFP-AtSYP41 and ST52-mRFP (PubMed=28339924).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Genetic integration: Method=Transfection/transduction; Gene=RGD; 3676; St6gal1 (Note=With p.Met53_Cys403del = ST52 Cyto+TM).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; O65359; Arabidopsis thaliana SYP41.
CC   Derived from site: In situ; Seedling; PO=PO_0008037.
CC   Breed/subspecies: Cultivar Bright Yellow 2.
OX   NCBI_TaxID=4097; ! Nicotiana tabacum (Common tobacco)
HI   CVCL_D5GM ! BY-2
CA   Spontaneously immortalized cell line
DT   Created: 02-05-24; Last updated: 10-04-25; Version: 3
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RX   PubMed=28339924; DOI=10.1093/pcp/pcx028;
RA   Ito, Yoko
RA   Toyooka, Kiminori
RA   Fujimoto, Masaru
RA   Ueda, Takashi
RA   Uemura, Tomohiro
RA   Nakano, Akihiko
RT   "The trans-Golgi network and the Golgi stacks behave independently
RT   during regeneration after brefeldin A treatment in tobacco BY-2
RT   cells.";
RL   Plant Cell Physiol. 58:811-821(2017).
//