ID   U2OS SP2 CL3
AC   CVCL_D4XZ
RX   PubMed=32436764;
CC   Population: Caucasian.
CC   Characteristics: Transfected with two constructs, one containing the PEX3 peroxisome targeting sequence (PTS) fused to RFP and FKBP (PTS-RFP-FKBP) and the other to the FKBP-rapamycin-binding (FRB) domain of mTOR linked to the N-terminal half of mouse BicD2 (BicD2N) and GFP (GFP-BicD2N-FRB) (PubMed=32436764).
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: HGNC; 3711; FKBP1A.
CC   Transfected with: HGNC; 3942; MTOR (with Met1_Arg2018del and Gln2114_Trp2549del = FRB).
CC   Transfected with: MGI; MGI:1924145; Bicd2 (with Arg401_Leu820del = Bicd2N).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 02-05-24; Last updated: 02-05-24; Version: 1
//
RX   PubMed=32436764; DOI=10.1177/2472555220920581;
RA   Vincent J., Preston M., Mouchet E., Laugier N., Corrigan A.,
RA   Boulanger J., Brown D.G., Clark R., Wigglesworth M., Carter A.P.,
RA   Bullock S.L.;
RT   "A high-throughput cellular screening assay for small-molecule
RT   inhibitors and activators of cytoplasmic dynein-1-based cargo
RT   transport.";
RL   SLAS Discovery 25:985-999(2020).
//