ID   Neuro2a-tNhtt-EGFP-150Q
AC   CVCL_D3YT
DR   RCB; RCB5514
DR   Wikidata; Q127382949
RX   PubMed=10574348;
CC   Characteristics: Transfected with the pVgRXR vector that contains the VgEcR fusion protein under the control of a CMV promoter and RXRA under the control of a RSV promoter as well as BleoR (from parent cell line).
CC   Characteristics: Transfected with a ecdysone inducible pIND-HDexon1-EGFP construct containing the first exon of HTT with a 150 Gln repeat followed by EGFP (PubMed=10574348).
CC   Genetic integration: Method=Transfection/transduction; Gene=FlyBase_Gene; FBgn0000546; EcR (Note=With p.Met1_Val221del, as 2nd part of VgECR).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:4851; HTT (Note=1st exon with 150-Q run).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:10477; RXRA.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Thr412del, as 1st part of VgECR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
CC   Cell type: Neural crest derived neuroblast; CL=CL_0002676.
CC   Breed/subspecies: A/J.
DI   NCIt; C21993; Mouse neuroblastoma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_D3XR ! Neuro2a-RXR
SX   Male
CA   Cancer cell line
DT   Created: 30-01-24; Last updated: 10-04-25; Version: 4
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RX   PubMed=10574348; DOI=10.1097/00001756-199908200-00001;
RA   Wang G.-H., Mitsui K., Kotliarova S., Yamashita A., Nagao Y.,
RA   Tokuhiro S., Iwatsubo T., Kanazawa I., Nukina N.;
RT   "Caspase activation during apoptotic cell death induced by expanded
RT   polyglutamine in N2a cells.";
RL   NeuroReport 10:2435-2438(1999).
//