ID   RT4 NF2.Q538P #8
AC   CVCL_D3Y6
DR   Wikidata; Q127384094
RX   PubMed=11448944;
CC   Characteristics: Expression of NF2 is doxycycline-inducible as the parent cell line was transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=11448944).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7773; NF2 (Note=With p.Gln538Pro).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: ChEBI; CHEBI_23995; N-ethyl-N-nitrosourea (ENU).
CC   Derived from site: In situ; Peripheral nervous system, sciatic nerve; UBERON=UBERON_0001322.
CC   Cell type: Schwann cell; CL=CL_0002573.
CC   Breed/subspecies: BDIX; RS=RS_0000091.
DI   NCIt; C60465; Rat malignant schwannoma
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_4006 ! RT4-D6P2T
CA   Cancer cell line
DT   Created: 30-01-24; Last updated: 10-04-25; Version: 4
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RX   PubMed=11448944; DOI=10.1093/hmg/10.14.1519;
RA   Gutmann D.H., Hirbe A.C., Haipek C.A.;
RT   "Functional analysis of neurofibromatosis 2 (NF2) missense
RT   mutations.";
RL   Hum. Mol. Genet. 10:1519-1529(2001).
//