ID   RT4-54
AC   CVCL_D3XY
SY   RT4-D6P2T clone 54
DR   Wikidata; Q127384097
RX   PubMed=11316791;
CC   Characteristics: Expression of NF2 is doxycycline-inducible as the parent cell line was transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=11316791).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7773; NF2.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: ChEBI; CHEBI_23995; N-ethyl-N-nitrosourea (ENU).
CC   Derived from site: In situ; Peripheral nervous system, sciatic nerve; UBERON=UBERON_0001322.
CC   Cell type: Schwann cell; CL=CL_0002573.
CC   Breed/subspecies: BDIX; RS=RS_0000091.
DI   NCIt; C60465; Rat malignant schwannoma
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_4006 ! RT4-D6P2T
CA   Cancer cell line
DT   Created: 30-01-24; Last updated: 10-04-25; Version: 4
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RX   PubMed=11316791; DOI=10.1101/gad.189601; PMCID=PMC312675;
RA   Morrison H., Sherman L.S., Legg J., Banine F., Isacke C., Haipek C.A.,
RA   Gutmann D.H., Ponta H., Herrlich P.;
RT   "The NF2 tumor suppressor gene product, merlin, mediates contact
RT   inhibition of growth through interactions with CD44.";
RL   Genes Dev. 15:968-980(2001).
//