ID   HepG2/8F_HS
AC   CVCL_D3XF
DR   RCB; RCB5677
DR   Wikidata; Q127381753
RX   PubMed=35406758;
CC   Population: Caucasian.
CC   Characteristics: Could be useful as a new cell source for pharmacological studies and constructing bioartificial liver systems. When heat-stimulated it expresses 8 liver-specific transcription factor genes and thus many liver functions are induced upon heat treatment (PubMed=35406758).
CC   Characteristics: Transduced with a pQMSCV-pHSP70B-TRE-pCMVmin-EGFP-IRES-tTA construct. The heat-activated HSPA6/HSP70B' promoter drives bicistronic gene expression of EGFP and of the tetracycline-controlled transactivator (tTA), which is mediated by an internal ribosomal entry site (IRES). Then, the tTA protein binds to TRE and activates the cytomegalovirus minimum promoter (pCMVmin) upstream of EGFP, which in turn induces further expression of the EGFP and tTA genes, providing a transcriptional positive feedback loop (from parent cell line).
CC   Characteristics: The amplified tTA promotes the expression of 8 liver-enriched transcription factor (LETF) genes that were transduced using two constructs: PB513B-1/TRE-HNF1A-FOXA2/HNF3B-CEBPA-CEBPB-CEBPG-EF1a-PuroR and PB513B-1/TRE-HNF1B-HNF4A-ONECUT1-EF1a-Bla (PubMed=35406758).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1833; CEBPA.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1834; CEBPB.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1837; CEBPG.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5022; FOXA2.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11621; HNF1A.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11630; HNF1B.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5024; HNF4A.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:8138; ONECUT1.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C3728; Hepatoblastoma
DI   ORDO; Orphanet_449; Hepatoblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D3XE ! HepG2-HSP
SX   Male
AG   15Y
CA   Cancer cell line
DT   Created: 30-01-24; Last updated: 10-04-25; Version: 4
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RX   PubMed=35406758; DOI=10.3390/cells11071194; PMCID=PMC8997820;
RA   Kitano H., Kawabe Y., Kamihira M.;
RT   "HepG2-based designer cells with heat-inducible enhanced liver
RT   functions.";
RL   Cells 11:1194.1-1194.16(2022).
//