ID   HepG2-HSP
AC   CVCL_D3XE
RX   PubMed=30962099;
CC   Population: Caucasian.
CC   Characteristics: Transduced with a pQMSCV-pHSP70B-TRE-pCMVmin-EGFP-IRES-tTA construct. The heat-activated HSPA6/HSP70B' promoter drives bicistronic gene expression of EGFP and of the tetracycline-controlled transactivator (tTA), which is mediated by an internal ribosomal entry site (IRES). Then, the tTA protein binds to TRE and activates the cytomegalovirus minimum promoter (pCMVmin) upstream of EGFP, which in turn induces further expression of the EGFP and tTA genes, providing a transcriptional positive feedback loop (PubMed=30962099).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C3728; Hepatoblastoma
DI   ORDO; Orphanet_449; Hepatoblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0027 ! Hep-G2
SX   Male
AG   15Y
CA   Cancer cell line
DT   Created: 30-01-24; Last updated: 30-01-24; Version: 1
//
RX   PubMed=30962099; DOI=10.1016/j.jbiosc.2019.03.008;
RA   Ito A., Teranishi R., Kamei K., Yamaguchi M., Ono A., Masumoto S.,
RA   Sonoda Y., Horie M., Kawabe Y., Kamihira M.;
RT   "Magnetically triggered transgene expression in mammalian cells by
RT   localized cellular heating of magnetic nanoparticles.";
RL   J. Biosci. Bioeng. 128:355-364(2019).
//