ID   2fTGH-U5A
AC   CVCL_D315
SY   U5A; U-5A
DR   BTO; BTO_0006556
DR   cancercelllines; CVCL_D315
DR   CancerTools; 151808
DR   ECACC; 12021506
DR   Wikidata; Q54584482
DR   Ximbio; 151808
RX   PubMed=7588638;
CC   Population: Caucasian.
CC   Characteristics: 2fTGH was derived from a HPRT negative subline of HT-1080. Expression of gpt is under the control of a IFN-alpha inducible IFI6 (6-16) promoter and is thus only expressed when IFN-alpha is present in the growth medium.
CC   Sequence variation: Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P0A9M5; Escherichia coli gpt.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transformant: ChEBI; CHEBI_132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
CC   Discontinued: ECACC; 12021506; true.
CC   Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
ST   Source(s): ECACC=12021506
ST   Amelogenin: X,Y
ST   CSF1PO: 12
ST   D13S317: 12,14
ST   D16S539: 9,12
ST   D5S818: 11,13
ST   D7S820: 9,10
ST   TH01: 6
ST   TPOX: 8
ST   vWA: 14,19
DI   NCIt; C3043; Fibrosarcoma
DI   ORDO; Orphanet_2030; Fibrosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0115 ! 2fTGH
SX   Male
AG   35Y
CA   Cancer cell line
DT   Created: 22-10-12; Last updated: 19-12-24; Version: 24
//
RX   PubMed=7588638; DOI=10.1002/j.1460-2075.1995.tb00192.x; PMCID=PMC394613;
RA   Lutfalla, Georges
RA   Holland, Sacha J.
RA   Cinato, Elisa
RA   Monneron, Daniele
RA   Reboul, Jerome
RA   Rogers, Neil C.
RA   Smith, Jon M.
RA   Stark, George Robert
RA   Gardiner, Katheleen
RA   Mogensen, Knud Erik
RA   Kerr, Ian Macpherson
RA   Uze, Gilles
RT   "Mutant U5A cells are complemented by an interferon-alpha beta
RT   receptor subunit generated by alternative processing of a new member
RT   of a cytokine receptor gene cluster.";
RL   EMBO J. 14:5100-5108(1995).
//