ID   BU3:N1
AC   CVCL_D2WI
DR   Wikidata; Q127380351
RX   PubMed=36332629;
CC   From: Boston University School of Medicine; Boston; USA.
CC   Population: Caucasian.
CC   Characteristics: Under the control of doxycycline expresses the intracellular domain of the NOTCH1 receptor (NICD1). Using ZFN a construct containing a reverse tetracycline-controlled transactivator (rtTA), NICD1 and puroR was inserted in the AAVS1 safe harbor locus (PubMed=36332629).
CC   Genetic integration: Method=ZFN; Gene=HGNC; HGNC:7881; NOTCH1 (Note=With p.Met1_Arg1762del = NICD1).
CC   Genetic integration: Method=ZFN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=ZFN; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=ZFN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D2WD ! BU3-10-Cr1
SX   Male
AG   32Y
CA   Induced pluripotent stem cell
DT   Created: 30-01-24; Last updated: 19-12-24; Version: 3
//
RX   PubMed=36332629; DOI=10.1016/j.stemcr.2022.10.007; PMCID=PMC9768581;
RA   Heinze D., Park S., McCracken A., Haratianfar M., Lindstrom J.,
RA   Villacorta-Martin C., Mithal A., Wang F.-Y., Yang M.-W., Murphy G.J.,
RA   Mostoslavsky G.;
RT   "Notch activation during early mesoderm induction modulates emergence
RT   of the T/NK cell lineage from human iPSCs.";
RL   Stem Cell Reports 17:2610-2628(2022).
//