ID   HeLa-MAGI
AC   CVCL_D259
SY   HeLa MAGI; HeLa Magi; MAGI; HeLa-CD4-LTR-beta-gal; HeLa-CD4-LTR/beta-gal; CD4-LTR/beta-gal; HeLa-Multinuclear Activation of a Galactosidase Indicator
DR   BTO; BTO_0003588
DR   CLO; CLO_0050934
DR   BEI_Resources; ARP-1470
DR   RCB; RCB2356
DR   Wikidata; Q54882719
RX   PubMed=1548759;
CC   Population: African American.
CC   Characteristics: Transfected with a construct pJK2 containing the HIV-1 LTR fused to a lacZ reporter gene that contains a MPKKKRK N-terminal sequence directing it to the nucleus (PubMed=1548759).
CC   Virology: Can be used to determine quantitatively the titer of laboratory-adapted HIV-1 strains (PubMed=1548759).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1678; CD4.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
ST   Source(s): RCB=RCB2356
ST   Amelogenin: X
ST   CSF1PO: 9,10
ST   D13S317: 12
ST   D16S539: 9,10
ST   D5S818: 11,12
ST   D7S820: 8,12
ST   TH01: 7
ST   TPOX: 8,12
ST   vWA: 16,18
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_E8AI ! HeLa-CD4
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 22-10-12; Last updated: 10-04-25; Version: 23
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RX   PubMed=1548759; DOI=10.1128/JVI.66.4.2232-2239.1992; PMCID=PMC289016;
RA   Kimpton J., Emerman M.;
RT   "Detection of replication-competent and pseudotyped human
RT   immunodeficiency virus with a sensitive cell line on the basis of
RT   activation of an integrated beta-galactosidase gene.";
RL   J. Virol. 66:2232-2239(1992).
//