ID   H9 AAVS1-TRE3G-NGN2 TEX264-/- Keima-REEP5
SY   H9 ES AAVS1-NGN2 TEX264-/-; PiggyBac-Keima-REEP5
RX   PubMed=37425907;
CC   Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
CC   Characteristics: Transduced with a PiggyBac transposon vector pAC150-PBLHL-4xHS-EF1a-Keima-REEP5 that localizes specifically to endoplasmic reticulum tubules (PubMed=37425907).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; 30247; TEX264.
CC   Transfected with: HGNC; 13805; NEUROG2.
CC   Transfected with: HGNC; 30077; REEP5.
CC   Transfected with: UniProtKB; Q1JV70; Montipora sp. 20 fluorescent protein mKeima.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 30-01-24; Last updated: 30-01-24; Version: 1
RX   PubMed=37425907; DOI=10.1101/2023.06.26.546565;
RA   Hoyer M.J., Smith I.R., Paoli J.C., Jiang Y.-Z., Paulo J.A.,
RA   Harper J.W.;
RT   "Combinatorial selective ER-phagy remodels the ER during
RT   neurogenesis.";
RL   bioRxiv 2023:06.26.546565-06.26.546565(2023).