ID   H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/-
AC   CVCL_D1ID
SY   H9 ES AAVS1-NGN2 FAM134C/A/B-/-;TEX264-/-; H9 FAM134A/B/C/TEX264-/-
DR   Wikidata; Q127381003
RX   PubMed=38429475;
CC   Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:25964; RETREG1.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:28450; RETREG2.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:27258; RETREG3.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:30247; TEX264.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13805; NEUROG2.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D1IC ! H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/-
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 30-01-24; Last updated: 19-12-24; Version: 3
CH   CVCL_D1IV ! H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/- Keima-RAMP4
CH   CVCL_D1IW ! H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/- Keima-REEP5
CH   CVCL_D1IE ! H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- RETREG1-/- TEX264-/- CCPG1-/-
//
RX   PubMed=38429475; DOI=10.1038/s41556-024-01356-4; PMCID=PMC10940164;
RA   Hoyer, Melissa J.
RA   Capitanio, Christina
RA   Smith, Ian R.
RA   Paoli, Julia C.
RA   Bieber, Anna
RA   Jiang, Yi-Zhi
RA   Paulo, Joao A.
RA   Gonzalez-Lozano, Miguel A.
RA   Baumeister, Wolfgang P.
RA   Wilfling, Florian
RA   Schulman, Brenda Arlene
RA   Harper, Jeffrey Wade
RT   "Combinatorial selective ER-phagy remodels the ER during
RT   neurogenesis.";
RL   Nat. Cell Biol. 26:378-392(2024).
//