ID   S2 PfRH5 variant 2
AC   CVCL_D1FU
SY   S2 PfRH5 v2.0
RX   PubMed=27457156;
CC   Group: Insect cell line.
CC   Group: Malaria research cell line.
CC   Transfected with: UniProtKB; Q8IFM5; Plasmodium falciparum (isolate 3D7) reticulocyte-binding protein homolog 5 (RH5) (PfRH5) (with p.Met1_Phe25del, p.Thr40Ala, p.Thr216Ala, p.Thr286Ala, p.Thr299Ala and a C-terminal His6 tag).
CC   Transfected with: FlyBase_Gene; FBgn0001218; Hsc70-3 (AA 1-18 = Signal sequence).
CC   Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_A8PY ! HighMan-S2
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 30-01-24; Last updated: 30-01-24; Version: 1
//
RX   PubMed=27457156; DOI=10.1038/srep30357;
RA   Hjerrild K.A., Jin J., Wright K.E., Brown R.E., Marshall J.M.,
RA   Labbe G.M., Silk S.E., Cherry C.J., Clemmensen S.B., Jorgensen T.,
RA   Illingworth J.J., Alanine D.G.W., Milne K.H., Ashfield R.,
RA   de Jongh W.A., Douglas A.D., Higgins M.K., Draper S.J.;
RT   "Production of full-length soluble Plasmodium falciparum RH5 protein
RT   vaccine using a Drosophila melanogaster Schneider 2 stable cell line
RT   system.";
RL   Sci. Rep. 6:30357-30357(2016).
//