ID   S2 PfRH5 variant 1
AC   CVCL_D1FT
SY   S2 PfRH5 v1.0
DR   Wikidata; Q127384143
RX   PubMed=27457156;
CC   Group: Insect cell line.
CC   Group: Malaria research cell line.
CC   Genetic integration: Method=Transfection/transduction; Gene=FlyBase_Gene; FBgn0001218; Hsc70-3 (Note=AA 1-18 = Signal sequence).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q8IFM5; Plasmodium falciparum (isolate 3D7) reticulocyte-binding protein homolog 5 (RH5) (PfRH5) (Note=With p.Met1_Phe25del, p.Thr40Ala, p.Cys203Tyr, p.Thr216Ala, p.Thr286Ala, p.Thr299Ala and a C-terminal His6 tag).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_A8PY ! HighMan-S2
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 30-01-24; Last updated: 10-04-25; Version: 3
//
RX   PubMed=27457156; DOI=10.1038/srep30357; PMCID=PMC4960544;
RA   Hjerrild K.A., Jin J., Wright K.E., Brown R.E., Marshall J.M.,
RA   Labbe G.M., Silk S.E., Cherry C.J., Clemmensen S.B., Jorgensen T.,
RA   Illingworth J.J., Alanine D.G.W., Milne K.H., Ashfield R.,
RA   de Jongh W.A., Douglas A.D., Higgins M.K., Draper S.J.;
RT   "Production of full-length soluble Plasmodium falciparum RH5 protein
RT   vaccine using a Drosophila melanogaster Schneider 2 stable cell line
RT   system.";
RL   Sci. Rep. 6:30357-30357(2016).
//