ID   AG-F
AC   CVCL_D101
SY   AGF
DR   ATCC; CRL-2530
DR   ATCC; CRL-11391
DR   BioSample; SAMN03151617
DR   BioSample; SAMN03471608
DR   cancercelllines; CVCL_D101
DR   Wikidata; Q54609413
RX   Patent=US5480825;
RX   PubMed=8255104;
RX   PubMed=12592342;
RX   PubMed=20143388;
RX   PubMed=29533902;
WW   http://www.cells-talk.com/index.php/page/copelibrary?key=AG-F
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
CC   Group: Patented cell line.
CC   Problematic cell line: Contaminated. Shown to be a CCRF-CEM derivative (PubMed=12592342; PubMed=20143388). Originally thought to originates from the bone marrow of a patient suffering fron neuroblastoma that evolved into a Hodgkin lymphoma.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00171.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-11391.
CC   Population: Caucasian.
CC   Doubling time: ~8-9 hours (Patent=US5480825).
CC   Sequence variation: Gene fusion; HGNC; 13222; BCL11B + HGNC; 2488; NKX2-5; Name(s)=NKX2.5-BCL11B (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 16712; FBXW7; Simple; p.Arg465Cys (c.1393C>T); ClinVar=VCV000376414; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3765; FLT3; Simple; p.Ala627Thr (c.1879G>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7881; NOTCH1; Simple; p.Leu1593_Arg1594insProArgLeuProHisAsnSerSerPheHisPheLeu (c.4780_4781ins36); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 7881; NOTCH1; Simple; p.Pro2412Thr (c.7234C>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg175His (c.524G>A); ClinVar=VCV000012374; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Heterozygous (from parent cell line).
CC   Discontinued: ATCC; CRL-2530; true.
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
CC   Cell type: T-cell; CL=CL_0000084.
ST   Source(s): ATCC
ST   Amelogenin: X
ST   CSF1PO: 10,11,12
ST   D13S317: 11,12
ST   D16S539: 10,13,14
ST   D5S818: 11,13,14,15
ST   D7S820: 8,14.1
ST   TH01: 6,7
ST   TPOX: 8,9
ST   vWA: 16,17,18,19
DI   NCIt; C7953; Childhood T acute lymphoblastic leukemia
DI   ORDO; Orphanet_99861; Precursor T-cell acute lymphoblastic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0207 ! CCRF-CEM
SX   Female
AG   3Y11M
CA   Cancer cell line
DT   Created: 22-10-12; Last updated: 05-10-23; Version: 27
//
RX   Patent=US5480825;
RA   Gazitt Y.;
RT   "AG-F human T cell line with unique phenotype and cytokine secretions.";
RL   Patent number US5480825, 02-Jan-1996.
//
RX   PubMed=8255104;
RA   Gazitt Y., He Y.-J., Shults K., Stelzer G.T., Cohen R.J.;
RT   "Isolation and characterization of an early T-helper/inducer cell line
RT   with a unique pattern of surface phenotype, constitutive cytokine
RT   secretion and myc oncogene expression.";
RL   Leukemia 7:2034-2044(1993).
//
RX   PubMed=12592342; DOI=10.1038/sj.leu.2402799;
RA   Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.;
RT   "False leukemia-lymphoma cell lines: an update on over 500 cell
RT   lines.";
RL   Leukemia 17:416-426(2003).
//
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A.,
RA   MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R.,
RA   Freshney R.I.;
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
//
RX   PubMed=29533902; DOI=10.1515/hsz-2017-0321;
RA   Drexler H.G., Pommerenke C., Eberth S., Nagel S.;
RT   "Hodgkin lymphoma cell lines: to separate the wheat from the chaff.";
RL   Biol. Chem. 399:511-523(2018).
//