ID   SNUe003-A-4
AC   CVCL_D0QP
SY   SNUhES3-TUBB3-mCherry; T-mC-KI-SE-3; IRMPe004-A-4
DR   BioSamples; SAMEA12504963
DR   hPSCreg; SNUe003-A-4
DR   Wikidata; Q123033546
RX   PubMed=36913849;
CC   From: Seoul National University; Seoul; South Korea.
CC   Population: Korean.
CC   Characteristics: Using CRISPR/Cas9 a T2A-mCherry-polyA-PGKp-Neo-polyA construct was introduced at the C-terminus of one allele of TUBB3 (PubMed=36913849).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9949 ! SNUhES3
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 05-10-23; Last updated: 10-09-24; Version: 3
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RX   PubMed=36913849; DOI=10.1016/j.scr.2023.103064;
RA   Kim A.-H., Lee H.M., Kim H.-S., Jung J., Seol H., Choi E., Lee S.,
RA   Choi Y.M., Jun J.K., Kim H.-S., Jang J.;
RT   "Establishment of TUBB3-mCherry knock-in human pluripotent stem cell
RT   line using CRISPR/Cas9 (SNUe003-A-4).";
RL   Stem Cell Res. 69:103064-103064(2023).
//