ID   HEK293/HF-Cas9-AAVS1
AC   CVCL_C9FA
DR   GeneCopoeia; SL553
DR   Wikidata; Q123031587
CC   Characteristics: Stably expresses a CMV-3xFLAG-HFCas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL553).
CC   Genetic integration: Method=Transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asn497Ala, p.Arg661Ala, p.Gln695Ala and p.Gln926Ala = High-fidelity Cas9).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 05-10-23; Last updated: 10-04-25; Version: 5
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