ID   T84/Cas9-hyg
AC   CVCL_C9EU
DR   GeneCopoeia; SL541
DR   Wikidata; Q123033602
CC   Population: Caucasian.
CC   Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL541).
CC   Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Leu1488Phefs*19 (c.4464delA); Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu542Lys (c.1624G>A); ClinVar=VCV000031944; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Lys340Asn (c.1020G>C); Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; c.560-1G>T; ClinVar=VCV003148249; Zygosity=Homozygous; Note=Splice acceptor mutation (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Derived from site: Metastatic; Lung; UBERON=UBERON_0002048.
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0555 ! T84
SX   Male
AG   72Y
CA   Cancer cell line
DT   Created: 05-10-23; Last updated: 10-04-25; Version: 5
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