ID   HEK293T-CRISPRa
AC   CVCL_C9DX
DR   BioGRID_ORCS_Cell_line; 1656
DR   Wikidata; Q123031592
RX   PubMed=34431042;
CC   Characteristics: Transduced with lentiviral vectors dCAS-VP64_Blast (EF1a-dCas9-VP64-Bsd-WPE) and MS2-P65-HSF1-Hygro (EF1a-MS2(N55K)-SV40(NLS)-RELA-HSF1-hygR-WPRE) (PubMed=34431042).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:5224; HSF1.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03612; Escherichia phage MS2 capsid protein (Note=With p.Asn56Lys).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Omics: Phenotyping; CRISPR screening.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 05-10-23; Last updated: 10-04-25; Version: 5
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RX   PubMed=34431042; DOI=10.1007/s11427-021-1990-5; PMCID=PMC8384091;
RA   Zhu S.-Y., Liu Y., Zhou Z., Zhang Z.-Y., Xiao X., Liu Z.-H., Chen A.,
RA   Dong X.-J., Tian F., Chen S.-H., Xu Y.-Y., Wang C.-H., Li Q.-H.,
RA   Niu X.-R., Pan Q., Du S., Xiao J.-Y., Wang J.-W., Wei W.-S.;
RT   "Genome-wide CRISPR activation screen identifies candidate receptors
RT   for SARS-CoV-2 entry.";
RL   Sci. China Life Sci. 65:701-717(2022).
//