ID   HCC70/Cas9-hyg
AC   CVCL_C9CI
DR   GeneCopoeia; SL544
DR   Wikidata; Q123031551
CC   Group: Triple negative breast cancer (TNBC) cell line.
CC   Population: African American.
CC   Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL544).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Derived from site: In situ; Breast; UBERON=UBERON_0000310.
CC   Cell type: Epithelial cell of mammary gland; CL=CL_0002327.
DI   NCIt; C4017; Breast ductal carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1270 ! HCC70
SX   Female
AG   49Y
CA   Cancer cell line
DT   Created: 05-10-23; Last updated: 10-04-25; Version: 5
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