ID   HEK293T/ACE2
AC   CVCL_C9BE
SY   HEK293T-hACE2
DR   BioGRID_ORCS_Cell_line; 1668
DR   GeneCopoeia; SL221
DR   Wikidata; Q123031593
RX   PubMed=35086559;
CC   Group: SARS-CoV-2 research cell line.
CC   Characteristics: Expresses ACE2 under the control of the CMV promoter (GeneCopoeia=SL221).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:13557; ACE2.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Omics: Phenotyping; CRISPR screening.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 05-10-23; Last updated: 10-04-25; Version: 5
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RX   PubMed=35086559; DOI=10.1186/s13073-022-01013-1; PMCID=PMC8792531;
RA   Grodzki M., Bluhm A.P., Schaefer M., Tagmount A., Russo M., Sobh A.,
RA   Rafiee R., Vulpe C.D., Karst S.M., Norris M.H.;
RT   "Genome-scale CRISPR screens identify host factors that promote human
RT   coronavirus infection.";
RL   Genome Med. 14:10.1-10.18(2022).
//