ID   Tau RD P301L FRET Sensitive Biosensor
AC   CVCL_C8YL
SY   HEK293T clone 18
DR   Wikidata; Q123033603
RX   PubMed=37553663;
CC   Characteristics: Transduced with a lentivirus construct EF1a-TauRD(P301L)-RLinker-mTurquoise2-T2A-TauRD(P301L)-RLinker-mNeonGreen-WPRE where TauRD(P301L) is the tau repeat domain (RD) with the disease-associated p.Pro301Leu (p.Pro636Leu) mutation and Rlinker is a (EAAAK)3 rigid linker (PubMed=37553663).
CC   Characteristics: Shows a high sensitivity in detecting bioactive tau seeds. These seeds can nucleate the aggregation of the reporter proteins. This aggregation produces a FRET signal (PubMed=37553663).
CC   Genetic integration: Method=Transduction; Gene=FPbase; ZRKRV; mNeonGreen (Note=Derivative of Branchiostoma lanceolatum LanYFP).
CC   Genetic integration: Method=Transduction; Gene=FPbase; 7AV5G; mTurquoise2 (Note=Tq2, cyan fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 05-10-23; Last updated: 10-09-24; Version: 3
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RX   PubMed=37553663; DOI=10.1186/s13024-023-00643-2; PMCID=PMC10408046;
RA   Lathuiliere A., Jo Y., Perbet R., Donahue C., Commins C., Quittot N.,
RA   Fan Z.-Y., Bennett R.E., Hyman B.T.;
RT   "Specific detection of Tau seeding activity in Alzheimer's disease
RT   using rationally designed biosensor cells.";
RL   Mol. Neurodegener. 18:53.1-53.13(2023).
//