ID   BHK-NS5B-FRLuc
AC   CVCL_C8QX
DR   Wikidata; Q123030789
RX   PubMed=20382106;
CC   Characteristics: Transfected with a (+)FLuc-(-)UTR-RLuc construct that comprises firefly luciferase (FLuc) and Renilla luciferase (RLuc) in reverse orientation flanked by both negative strands of the HCV 5' and 3' UTRs. The FLuc and RLuc reporter proteins are respectively regulated by host polymerase and NS5B polymerase (PubMed=20382106).
CC   Characteristics: Transfected with hepatitis C virus NS5B (a RNA-dependent RNA polymerase).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=10036; ! Mesocricetus auratus (Golden hamster)
HI   CVCL_C8QW ! BHK-NS5B
SX   Male
AG   1D
CA   Spontaneously immortalized cell line
DT   Created: 05-10-23; Last updated: 10-04-25; Version: 4
//
RX   PubMed=20382106; DOI=10.1016/j.ab.2010.04.004;
RA   Lee J.-C., Tseng C.-K., Chen K.-J., Huang K.-J., Lin C.-K., Lin Y.-T.;
RT   "A cell-based reporter assay for inhibitor screening of hepatitis C
RT   virus RNA-dependent RNA polymerase.";
RL   Anal. Biochem. 403:52-62(2010).
//