ID   HeLa HaloTag-LC3/ATG3-K62D/K64D-mCherry
AC   CVCL_C8QM
DR   Wikidata; Q123031612
RX   DOI=10.1101/2023.07.17.549391;
CC   Population: African American.
CC   Characteristics: Stably expresses HaloTag-LC3 to measure autophagic flux. Also expresses an internally mCherry-tagged ATG3 with p.Lys62Asp and p.Lys64Asp.
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:20962; ATG3.
CC   Genetic integration: Method=Transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:20962; ATG3 (Note=With p.Lys62Asp, p.Lys64Asp and a mCherry reporter inserted between Glu-125 and Ile-126).
CC   Genetic integration: Method=Transduction; Gene=RGD; 621315; Map1lc3b.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Caution: While described in the preprint DOI=10.1101/2023.07.17.549391, this cell line is no longer used as it has been found not be effective (personal communication of Ren, Xuefeng).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C8QC ! HeLa HaloTag-LC3
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 05-10-23; Last updated: 10-04-25; Version: 6
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RX   DOI=10.1101/2023.07.17.549391;
RA   Rao S.-L., Strong L.M., Ren X.-F., Skulsuppaisarn M., Lazarou M.,
RA   Hurley J.H., Hummer G.;
RT   "Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the
RT   phagophore.";
RL   bioRxiv 2023:07.17.549391-07.17.549391(2023).
//