ID   HEKExpress
AC   CVCL_C8CE
DR   Wikidata; Q123031596
RX   PubMed=30591923;
RX   PubMed=37232541;
WW   Provider; ExcellGene; -; https://excellgene.com/technologies/hek293
CC   Group: Serum/protein free medium cell line.
CC   Characteristics: Grows in suspension growth conditions in animal component-free media.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
CC   Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Caution: This cell line is incorrectly described as being derived from HEK293 (instead of HEK293T) in PubMed=30591923.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 29-06-23; Last updated: 14-08-25; Version: 6
CH   CVCL_F0B4 ! HEK2Express
CH   CVCL_C8CF ! HEKzeroT
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RX   PubMed=37232541; DOI=10.1002/bit.28414;
RA   Croissant, Coralie
RA   Armitano, Joshua
RA   Lazuech, Bertrand
RA   Svec, Danijel
RA   Pugin, Cyril
RA   Guesdon, Anais
RA   Bryan, Louise
RA   Castro, Antonio
RA   Neuhaus, Lea
RA   Fonti, Giulia
RA   Martinis, Jacopo
RA   Wurm, Maria Joao
RA   Wurm, Florian Maria
RA   Pino, Paco
RT   "A new T-antigen negative HEK293 cell line with improved AAV
RT   productivity.";
RL   Biotechnol. Bioeng. 120:1953-1960(2023).
//
RX   PubMed=30591923; DOI=10.1016/j.omtm.2018.11.004; PMCID=PMC6305802;
RA   Blessing, Daniel
RA   Vachey, Gabriel
RA   Pythoud, Catherine
RA   Rey, Maria
RA   Padrun, Vivianne
RA   Wurm, Florian Maria
RA   Schneider, Bernard L.
RA   Deglon, Nicole
RT   "Scalable production of AAV vectors in orbitally shaken HEK293
RT   cells.";
RL   Mol. Ther. Methods Clin. Dev. 13:14-26(2019).
//