ID   WAe009-A-H
AC   CVCL_C7FS
SY   H9-NKX2.5GFP/TBX5Td-T2A-18; SKLRMe004-A
DR   hPSCreg; WAe009-A-H
DR   Wikidata; Q117704873
RX   PubMed=36608451;
CC   From: State Key Laboratory of Reproductive Medicine, Nanjing Medical University; Nanjing; China.
CC   Characteristics: Using CRISPR/Cas9 two constructs T2A-EGFP-PGK-PuroR and T2A-Tdtomato-EF1a-NeoR were respectively inserted at the C-terminus of NKX2.5 and TBX5 (PubMed=36608451).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 21-03-23; Last updated: 05-10-23; Version: 3
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RX   PubMed=36608451; DOI=10.1016/j.scr.2023.103015;
RA   He Z.-Z., Sun H., Yu T.-Y., Yang Y.-H., Zhang X.-Q., Yang Y.;
RT   "Generation of a human embryonic stem cell line (SKLRMe004-A) carrying
RT   NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters.";
RL   Stem Cell Res. 67:103015-103015(2023).
//