ID   MSOD
AC   CVCL_C6VW
SY   M-SOD; Mesenchymal Sword of Damocles; hTERT-iDS; 196hT-iDS clone 20
DR   Wikidata; Q117704716
RX   Patent=WO2013186264A1;
RX   PubMed=24561906;
RX   PubMed=34486186;
CC   Group: Patented cell line.
CC   Characteristics: Capable to efficiently differentiate toward the osteogenic lineage (PubMed=24561906).
CC   Characteristics: Transduced with a lentiviral vector containing hTERT and eGFP respectively under the control of the CMV and EF1a promoters and a retro-vector containing iCas9-2A-CD19t under the control of a CMV promoter (PubMed=24561906).
CC   Characteristics: The suicide system of this cell line can be activated by addition of an homodimerizer that activates the apoptosis pathway through the dimerization of iCas9 (PubMed=24561906).
CC   Transfected with: HGNC; 1633; CD19 (with p.Lys324_Arg556del = CD19t).
CC   Transfected with: HGNC; 11730; TERT.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
CC   Cell type: Mesenchymal stem cell of bone marrow; CL=CL_0002540.
ST   Source(s): PubMed=24561906
ST   Amelogenin: X
ST   CSF1PO: 10,11
ST   D13S317: 12,14
ST   D16S539: 12
ST   D18S51: 12,17
ST   D21S11: 30,32.2
ST   D3S1358: 16,17
ST   D5S818: 11,12
ST   D7S820: 12
ST   D8S1179: 13,15
ST   FGA: 21,23
ST   Penta D: 11,13
ST   Penta E: 10,14
ST   TH01: 9.3
ST   TPOX: 8
ST   vWA: 15,16
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C6VV ! 196hT
SX   Female
AG   51Y
CA   Telomerase immortalized cell line
DT   Created: 21-03-23; Last updated: 29-06-23; Version: 2
//
RX   Patent=WO2013186264A1;
RA   Bourgine P.-E.;
RT   "Immortalized mesenchymal stem cells that can be killed through an
RT   inducible apoptosis system.";
RL   Patent number WO2013186264A1, 19-Dec-2013.
//
RX   PubMed=24561906; DOI=10.1016/j.scr.2013.12.006;
RA   Bourgine P.-E., Le Magnen C., Pigeot S., Geurts J., Scherberich A.,
RA   Martin I.;
RT   "Combination of immortalization and inducible death strategies to
RT   generate a human mesenchymal stromal cell line with controlled
RT   survival.";
RL   Stem Cell Res. 12:584-598(2014).
//
RX   PubMed=34486186; DOI=10.1002/adma.202103737;
RA   Pigeot S., Klein T., Gullotta F., Dupard S.J., Garcia-Garcia A.,
RA   Garcia-Garcia A., Prithiviraj S., Lorenzo P., Filippi M., Jaquiery C.,
RA   Kouba L., Asnaghi M.A., Raina D.B., Dasen B., Isaksson H.,
RA   Onnerfjord P., Tagil M., Bondanza A., Martin I., Bourgine P.-E.;
RT   "Manufacturing of human tissues as off-the-shelf grafts programmed to
RT   induce regeneration.";
RL   Adv. Mater. 33:e2103737.1-e2103737.13(2021).
//