ID   LUMCi004-A-1
AC   CVCL_C6UC
SY   LU99_AAVS1-bxb-v2
DR   BioSamples; SAMEA112164540
DR   hPSCreg; LUMCi004-A-1
DR   Wikidata; Q117704634
RX   PubMed=36495703;
CC   From: Leiden University Medical Center; Leiden; Netherlands.
CC   Population: Caucasian.
CC   Characteristics: Contains a platform to precisely integrate DNA payloads into the cell genome. Using CRISPR/Cas9 a STRAIGHT-IN v2 construct (loxP-EBFP2-attP(bxb1)-BleoR-lox257) was introduced in one of the alleles of the AAVS1 safe harbor locus (PubMed=36495703).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Ser30Arg, p.Tyr39Asn, p.Phe64Leu, p.Tyr66His, p.Ser72Ala, p.Asn105Thr, p.Ile128Val, p.Tyr145Phe, p.Val150Ile, p.Asp155Val, p.Ile171Val, p.Asn198Ser, p.Ala206Val, p.Val224Arg, p.His231Leu = EBFP2).
CC   Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Skin; UBERON=UBERON_0002097.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_UK77 ! LUMCi004-A
SX   Female
AG   30-34Y
CA   Induced pluripotent stem cell
DT   Created: 21-03-23; Last updated: 29-06-23; Version: 2
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RX   PubMed=36495703; DOI=10.1016/j.scr.2022.102991;
RA   Blanch-Asensio A., van der Vaart B., Vinagre M., Groen E.,
RA   Arendzen C.H., Freund C.M.A.H., Geijsen N., Mummery C.L., Davis R.P.;
RT   "Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC
RT   lines for integrating DNA payloads.";
RL   Stem Cell Res. 66:102991-102991(2023).
//