ID   WAe009-A-99
AC   CVCL_C6TU
SY   dCas9-p300 H9 21; dCas9-p300 H9 clone 21; KITe010-A-99
DR   BioSamples; SAMEA111365973
DR   hPSCreg; WAe009-A-99
DR   Wikidata; Q117704976
RX   PubMed=36493708;
CC   From: Korea Institute of Toxicology; Daejeon; South Korea.
CC   Characteristics: Transduced with a dCas9-p300-P2A-PuroR construct where the dCas9 is fused to the catalytic HAT core domain of p300 (PubMed=36493708).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3373; EP300 (Note=With p.Met1_Lys1047del and p.Arg1665_His2414del = catalytic domain).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 21-03-23; Last updated: 14-08-25; Version: 7
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RX   PubMed=36493708; DOI=10.1016/j.scr.2022.102986;
RA   Kang, Eun-Hye
RA   Kim, Ji-Woo
RA   Noh, Haneul
RA   Park, Seong-Hoon
RA   Park, Han-Jin
RA   Yoon, Seokjoo
RA   Kim, Hyemin
RT   "Establishment of a human embryonic stem cell line, WAe009-A-99, with
RT   constitutive expression of the dCas9-p300 fusion protein.";
RL   Stem Cell Res. 66:102986-102986(2023).
//