ID   A549 Safe Harbor Landing Pad
AC   CVCL_C3EV
DR   cancercelllines; CVCL_C3EV
DR   Wikidata; Q114310530
WW   https://www.sigmaaldrich.com/catalog/product/sigma/cll1220
CC   Population: Caucasian.
CC   Characteristics: Using ZFN a fluorescent mKATE2 tag flanked by Cre-lox sites (LoxN-EF1a-Lox2272-mKATE2-LoxP) was introduced in the AAVS1 safe harbor locus. The mKATE2 tag can be exchanged for a payload of the user's choice using Cre recombinase and a targeting vector with appropriate lox sites (Sigma-Aldrich).
CC   Doubling time: ~22 hours (Sigma-Aldrich).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
CC   Transfected with: mKate2, a bright red fluorescent protein derived from eqFP578.
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
DI   NCIt; C3512; Lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0023 ! A-549
SX   Male
AG   58Y
CA   Cancer cell line
DT   Created: 22-09-22; Last updated: 05-10-23; Version: 5
//