ID   U2OS BFP-LMNB1 GFP-TUBA1B RFP-ACTB
AC   CVCL_C3C9
SY   U2OS BFP-LMNB1/GFP-TUBA1B/RFP-ACTB; U2OS LMNB1-TUBA1B-ACTB
DR   Sigma-Aldrich; CLL1218
DR   Wikidata; Q114313146
CC   Population: Caucasian.
CC   Characteristics: Edited by ZFN at the LMNB1 locus to insert a TagBFP construct at the N-terminus of the gene, at the TUB1A1B locus to insert a TagGFP2 construct at the N-terminus of the gene and at the ACTB locus to insert a TagRFP construct at the N-terminus of the gene (Sigma-Aldrich=CLL1218).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=ZFN; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578).
CC   Genetic integration: Method=ZFN; Gene=FPbase; GJETS; TagGFP2 (Note=Green fluorescent protein derived from Aequorea macrodactyla GFP).
CC   Genetic integration: Method=ZFN; Gene=FPbase; S4HC8; TagRFP (Note=Red fluorescent protein derived from eqFP578).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C3CB ! U2OS BFP-LMNB1 RFP-ACTB
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 22-09-22; Last updated: 10-04-25; Version: 8
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