ID   U2OS BFP-LMNB1 GFP-TUBA1B RFP-ACTB
AC   CVCL_C3C9
SY   U2OS BFP-LMNB1/GFP-TUBA1B/RFP-ACTB; U2OS LMNB1-TUBA1B-ACTB
DR   Wikidata; Q114313146
WW   https://www.sigmaaldrich.com/catalog/product/sigma/cll1218
CC   Population: Caucasian.
CC   Characteristics: Edited by ZFN at the LMNB1 locus to insert a TagBFP construct at the N-terminus of the gene, at the TUB1A1B locus to insert a TagGFP2 construct at the N-terminus of the gene and at the ACTB locus to insert a TagRFP construct at the N-terminus of the gene.
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: UniProtKB; Q8WTC6; Aequorea macrodactyla GFP (with p.Lys3Gly, p.Thr9Ala, p.Ser65Cys, p.Phe99Gln, p.Met128Lys, p.Asn144Ser, p.Met153Arg, p.Lys162Glu, p.Val163Ala, p.Ile167Thr, p.Ala206Lys, p.Thr214Ala, p.Phe220Leu, p.Phe223Ser, p.Gly228Cys and Lys238Arg = TagGFP2).
CC   Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the BFP mTagBFP).
CC   Transfected with: UniProtKB; D0VX33; Entacmaea quadricolor FP480 (with p.Thr6Lys, p.Gln41Met, p.His67Ser, p.Gln134Lys, p.Ala142Pro, p.His143Ser, p.Met146Lys, p.Tyr148Lys, p.Ala158Val, p.Leu160Met, p.Tyr197Arg, p.Arg201Ile, p.Val211Trp and p.Cys222Ser = TagRFP).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C3CB ! U2OS BFP-LMNB1 RFP-ACTB
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 22-09-22; Last updated: 05-10-23; Version: 5
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