ID   RUESe002-A-5
AC   CVCL_C1W5
SY   RUES2-RFP-SMAD1
DR   BioSamples; SAMEA10794729
DR   hPSCreg; RUESe002-A-5
DR   Wikidata; Q114312932
CC   From: Rockefeller University; New York; USA.
CC   Characteristics: Using CRISPR/Cas9 a Bsd-P2A-tagRFP construct was introduced at the N-terminal end of SMAD1.
CC   Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Transfected with: UniProtKB; D0VX33; Entacmaea quadricolor FP480 (with p.Thr6Lys, p.Gln41Met, p.His67Ser, p.Gln134Lys, p.Ala142Pro, p.His143Ser, p.Met146Lys, p.Tyr148Lys, p.Ala158Val, p.Leu160Met, p.Tyr197Arg, p.Arg201Ile, p.Val211Trp and p.Cys222Ser = TagRFP).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B810 ! RUES2
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 22-09-22; Last updated: 29-06-23; Version: 4
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