ID   HEK293T Tet-off FLTau
AC   CVCL_C1CR
SY   Tet-FLTau
DR   Wikidata; Q114311580
RX   PubMed=36732333;
CC   Characteristics: Expresses Tau/MAPT under the control of a Tet-regulated promoter (PubMed=36732333).
CC   Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:6893; MAPT (Note=With p.Pro301Leu and p.Val337Met).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0063 ! HEK293T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 22-09-22; Last updated: 10-04-25; Version: 9
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RX   PubMed=36732333; DOI=10.1038/s41467-023-36058-2; PMCID=PMC9894937;
RA   Saha I., Yuste-Checa P., Da Silva Padilha M., Guo Q., Korner R.,
RA   Holthusen H., Trinkaus V.A., Dudanova I., Fernandez-Busnadiego R.,
RA   Baumeister W.P., Sanders D.W., Gautam S., Diamond M.I., Hartl F.-U.,
RA   Hipp M.S.;
RT   "The AAA+ chaperone VCP disaggregates Tau fibrils and generates
RT   aggregate seeds in a cellular system.";
RL   Nat. Commun. 14:560.1-560.17(2023).
//