ID   3T3J2NHP
AC   CVCL_C0R6
DR   Wikidata; Q112926974
RX   PubMed=10648628;
CC   Characteristics: Used a feeder cells to support transduced keratinocyte populations during selection with one of G418, hygromycin or puromycin.
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: Swiss albino.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_W667 ! 3T3-J2
SX   Male
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 23-06-22; Last updated: 05-10-23; Version: 5
//
RX   PubMed=10648628; DOI=10.1128/mcb.20.4.1436-1447.2000;
RA   Dickson M.A., Hahn W.C., Ino Y., Ronfard V., Wu J.Y., Weinberg R.A.,
RA   Louis D.N., Li F.P., Rheinwald J.G.;
RT   "Human keratinocytes that express hTERT and also bypass a
RT   p16(INK4a)-enforced mechanism that limits life span become immortal yet
RT   retain normal growth and differentiation characteristics.";
RL   Mol. Cell. Biol. 20:1436-1447(2000).
//