ID   PC12-27
SY   PC 12-27; PC12 clone 27
DR   TOKU-E; 3553
DR   Wikidata; Q112930276
RX   PubMed=1657914;
RX   PubMed=8900203;
RX   PubMed=9856988;
RX   PubMed=9933147;
RX   PubMed=10517799;
RX   PubMed=12106430;
CC   Characteristics: Fully incompetent for both branches of regulated secretion, those of dense granules and synaptic-like microvesicles, possibly because of the impairment of a general expression control system that appears to operate independently of neuroendocrine cell differentiation (PubMed=8900203).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Adrenal gland; UBERON=UBERON_0002369.
CC   Breed/subspecies: NEDH.
DI   NCIt; C121568; Rat adrenal gland pheochromocytoma
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_0481 ! PC12
SX   Male
AG   Age unspecified
CA   Cancer cell line
DT   Created: 23-06-22; Last updated: 29-06-23; Version: 5
RX   PubMed=1657914; DOI=10.1016/S0021-9258(18)54903-6;
RA   Zacchetti D., Clementi E., Fasolato C., Lorenzon P., Zottini M.,
RA   Grohovaz F., Fumagalli G., Pozzan T., Meldolesi J.;
RT   "Intracellular Ca2+ pools in PC12 cells. A unique, rapidly exchanging
RT   pool is sensitive to both inositol 1,4,5-trisphosphate and
RT   caffeine-ryanodine.";
RL   J. Biol. Chem. 266:20152-20158(1991).
RX   PubMed=8900203; DOI=10.1074/jbc.271.43.27116;
RA   Corradi N., Borgonovo B., Clementi E., Bassetti M., Racchetti G.,
RA   Consalez G.G., Huttner W.B., Meldolesi J., Rosa P.;
RT   "Overall lack of regulated secretion in a PC12 variant cell clone.";
RL   J. Biol. Chem. 271:27116-27124(1996).
RX   PubMed=9856988; DOI=10.1074/jbc.273.52.34683;
RA   Borgonovo B., Racchetti G., Malosio M.L., Benfante R., Podini P.,
RA   Rosa P., Meldolesi J.;
RT   "Neurosecretion competence, an independently regulated trait of the
RT   neurosecretory cell phenotype.";
RL   J. Biol. Chem. 273:34683-34686(1998).
RX   PubMed=9933147; DOI=10.1007/pl00005319;
RA   Arslan G., Kull B., Fredholm B.B.;
RT   "Signaling via A2A adenosine receptor in four PC12 cell clones.";
RL   Naunyn Schmiedebergs Arch. Pharmacol. 359:28-32(1999).
RX   PubMed=10517799; DOI=10.1111/j.1469-7793.1999.t01-1-00043.x;
RA   Malosio M.L., Benfante R., Racchetti G., Borgonovo B., Rosa P.,
RA   Meldolesi J.;
RT   "Neurosecretory cells without neurosecretion: evidence of an
RT   independently regulated trait of the cell phenotype.";
RL   J. Physiol. (Lond.) 520:43-52(1999).
RX   PubMed=12106430; DOI=10.1111/j.1460-9568.1992.tb00121.x;
RA   Clementi E., Racchetti G., Zacchetti D., Panzeri M.C., Meldolesi J.;
RT   "Differential expression of markers and activities in a group of PC12
RT   nerve cell clones.";
RL   Eur. J. Neurosci. 4:944-953(1992).