ID   PZ-TR
AC   CVCL_C0NN
SY   Clone 4
DR   cancercelllines; CVCL_C0NN
DR   Wikidata; Q114312744
RX   PubMed=26208032;
CC   Population: Caucasian.
CC   Biotechnology: Used for as reporter cell line for the assessment of thyroid receptor (TR) transcriptional activity. Triiodothyronine (T3) induced luciferase activity in a dose-dependent manner, and the sensitivity of assay allowed for the detection of nanomolar T3 concentrations (PubMed=26208032).
CC   Characteristics: Expression of luciferase is under the control of a promoter containing two thyroid hormone response elements (TREs) separated by four nucleotides (DR4) followed by a SV40 promoter (PubMed=26208032).
CC   Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: UniProtKB; P08659; Firefly luciferase.
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C3728; Hepatoblastoma
DI   ORDO; Orphanet_449; Hepatoblastoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0027 ! Hep-G2
SX   Male
AG   15Y
CA   Cancer cell line
DT   Created: 23-06-22; Last updated: 05-10-23; Version: 5
//
RX   PubMed=26208032; DOI=10.1021/acs.jafc.5b01519;
RA   Illes P., Brtko J., Dvorak Z.;
RT   "Development and characterization of a human reporter cell line for
RT   the assessment of thyroid receptor transcriptional activity: a case of
RT   organotin endocrine disruptors.";
RL   J. Agric. Food Chem. 63:7074-7083(2015).
//