ID   ESIBIe003-A-10
AC   CVCL_C0CN
SY   HES3 INS-GFP GCG-mCh; INSGFP/wGCGmCh/w hESC; MCRIe004-A-10
DR   BioSamples; SAMEA9462434
DR   hPSCreg; ESIBIe003-A-10
DR   Wikidata; Q112929540
RX   PubMed=34619644;
CC   From: Murdoch Children's Research Institute; Melbourne; Australia.
CC   Characteristics: Reporter cell line that expresses GFP and mCherry fluorescent proteins under the control of the endogenous insulin (INS) and glucagon (GCG) loci respectively. Using homologous recombination an EGFP reporter gene was introduced in one allele of the INS locus. Using TALEN a mCherry reporter was introduced at the N-terminus in one allele of the GCG locus (PubMed=34619644).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
CC   Omics: SNP array analysis.
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_XA15 ! HES-3 INSGFP/w
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 23-06-22; Last updated: 29-06-23; Version: 4
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RX   PubMed=34619644; DOI=10.1016/j.scr.2021.102547;
RA   Labonne T., Elefanty A.G., Stanley E.G., Schiesser J.V.;
RT   "An INSULIN-GFP/GLUCAGON-mCherry reporter line for the study of human
RT   pancreatic endocrine cell development.";
RL   Stem Cell Res. 56:102547-102547(2021).
//