ID   SRD-14
AC   CVCL_B7PA
SY   Sterol Regulatory Defective-14
DR   Wikidata; Q112930417
RX   PubMed=15247248;
RX   PubMed=15866869;
CC   Selected for resistance to: ChEBI; CHEBI:77317; 4-[2,2-bis(diethoxyphosphoryl)ethenyl]-2,6-ditert-butylphenol] (SR12813).
CC   Sequence variation: Gene deletion; UniProtKB; Q8CFA6; Insig1; Zygosity=Heterozygous; Note=The 2nd allele has a loss of function (PubMed=15247248).
CC   Transformant: NCIt; C129643; Cesium-137 gamma radiation.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_D893 ! CHO-7
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 23-06-22; Last updated: 30-01-24; Version: 5
//
RX   PubMed=15247248; DOI=10.1074/jbc.M406406200;
RA   Sever N., Lee P.C.W., Song B.-L., Rawson R.B., DeBose-Boyd R.A.;
RT   "Isolation of mutant cells lacking Insig-1 through selection with
RT   SR-12813, an agent that stimulates degradation of
RT   3-hydroxy-3-methylglutaryl-coenzyme A reductase.";
RL   J. Biol. Chem. 279:43136-43147(2004).
//
RX   PubMed=15866869; DOI=10.1074/jbc.M502989200;
RA   Lee P.C.W., Sever N., DeBose-Boyd R.A.;
RT   "Isolation of sterol-resistant Chinese hamster ovary cells with
RT   genetic deficiencies in both Insig-1 and Insig-2.";
RL   J. Biol. Chem. 280:25242-25249(2005).
//