ID   iSLK.219
AC   CVCL_B6YV
SY   iSLK.219 clone #10
DR   Wikidata; Q111733519
RX   PubMed=21419799;
RX   PubMed=22987579;
CC   Problematic cell line: Contaminated. Shown to be a Caki-1 derivative (PubMed=22987579). The grand-parent cell line was originally thought to originate from a 28 year old male patient with HIV-1 negative Kaposi sarcoma.
CC   Population: Caucasian.
CC   Characteristics: rKSHV.219 includes a RFP-GFP-PuroR construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF1a promoter and PuroR expressed by the RSV promoter.
CC   Characteristics: Transduced with pRetroX-Tet-On that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and with RetroX-Tight-Hyg-RTA.
CC   Virology: Maintains latent rKSHV.219 as indicated by expression of constitutively expressed (eGFP) but not a lytic phase (RFP) reporter gene. When exposed to either sodium butyrate or doxycycline, the cells are activated to lytic replication and express RFP and KSHV lytic genes and release large quantities of infectious virus (PubMed=21419799).
CC   Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219.
CC   Sequence variation: Mutation; HGNC; HGNC:9884; RB1; Simple; p.Leu98Pro (c.293T>C); ClinVar=VCV001502192; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: Metastatic; Skin; UBERON=UBERON_0002097.
ST   Source(s): PubMed=22987579
ST   Amelogenin: X
ST   CSF1PO: 11
ST   D13S317: 11,12
ST   D16S539: 12
ST   D5S818: 11
ST   D7S820: 8,12
ST   TH01: 6,8
ST   TPOX: 8,11
ST   vWA: 15,17
DI   NCIt; C4033; Clear cell renal cell carcinoma
DI   ORDO; Orphanet_319276; Clear cell renal carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B6YU ! iSLK
SX   Male
AG   49Y
CA   Cancer cell line
DT   Created: 17-03-22; Last updated: 14-08-25; Version: 10
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RX   PubMed=21419799; DOI=10.1016/j.jviromet.2011.03.012; PMCID=PMC3095772;
RA   Myoung, Jinjong
RA   Ganem, Donald Emil
RT   "Generation of a doxycycline-inducible KSHV producer cell line of
RT   endothelial origin: maintenance of tight latency with efficient
RT   reactivation upon induction.";
RL   J. Virol. Methods 174:12-21(2011).
//
RX   PubMed=22987579; DOI=10.1002/ijc.27849;
RA   Sturzl, Michael
RA   Gaus, Dominika
RA   Dirks, Wilhelm Gerhard
RA   Ganem, Donald Emil
RA   Jochmann, Ramona
RT   "Kaposi's sarcoma-derived cell line SLK is not of endothelial origin,
RT   but is a contaminant from a known renal carcinoma cell line.";
RL   Int. J. Cancer 132:1954-1958(2013).
//