ID   NIH/3T3-174
AC   CVCL_B6J0
DR   Wikidata; Q112129459
RX   Patent=RU2396343;
RX   PubMed=28873013;
CC   Group: Patented cell line.
CC   Characteristics: Overexpresses SURF6 in the presence of doxycycline.
CC   Characteristics: Transfected with two constructs: 1) pcDNA3.1(-)rtTA which contains a rtTA and a neo gene. The reverse tetracycline-controlled transactivator (rtTA) is a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. 2) pBI-mSURF6 that contains a bidirectorial tetracycline-responsible element (Tre) flanked by two minimal bi-directorial CMV promoters which govern co-expression of SURF6 and EGFP.
CC   Transfected with: MGI; MGI:98447; Surf6.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: NIH Swiss.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0594 ! NIH 3T3
SX   Male
AG   Embryo
CA   Transformed cell line
DT   Created: 17-03-22; Last updated: 29-06-23; Version: 5
//
RX   Patent=RU2396343;
RA   Polzikov M.A., Zatsepina O.V.;
RT   "Genetically modified mouse fibroblast line NIH/3T3-174.";
RL   Patent number RU2396343, 10-Aug-2010.
//
RX   PubMed=28873013; DOI=10.1080/15384101.2017.1371880;
RA   Moraleva A., Magoulas C., Polzikov M.A., Hacot S., Mertani H.C.,
RA   Diaz J.-J., Zatsepina O.V.;
RT   "Involvement of the specific nucleolar protein SURF6 in regulation of
RT   proliferation and ribosome biogenesis in mouse NIH/3T3 fibroblasts.";
RL   Cell Cycle 16:1979-1991(2017).
//