ID   OUMS-29/TK
AC   CVCL_B6EK
SY   OUMS-29/thymidine kinase
DR   Wikidata; Q112129481
RX   PubMed=11575821;
CC   Population: Japanese.
CC   Characteristics: The introduction of HSV-1 TK gene makes the cell line 100x as sensitive to ganciclovir (GCV) compared with unmodified cells. This suicide gene can be used as a safeguard in the use of this cell line in hybrid artificial liver (PubMed=11575821).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q9QNF7; Human herpesvirus 1 TK.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
CC   Derived from site: In situ; Fetal liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatocyte; CL=CL_0000182.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9130 ! OUMS-29
SX   Male
AG   21FW
CA   Transformed cell line
DT   Created: 17-03-22; Last updated: 10-04-25; Version: 7
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RX   PubMed=11575821; DOI=10.1097/00002480-200109000-00016;
RA   Kobayashi N., Miyazaki M., Westerman K.A., Noguchi H., Sakaguchi M.,
RA   Totsugawa T., Watanabe T., Matsumura T., Fujiwara T., Leboulch P.,
RA   Tanaka N., Namba M.;
RT   "Construction of a differentiated human hepatocyte cell line
RT   expressing the herpes simplex virus-thymidine kinase gene.";
RL   ASAIO J. 47:476-480(2001).
//