ID   Hepa/HS
AC   CVCL_B5ZG
DR   Wikidata; Q111733353
RX   PubMed=34149171;
CC   Characteristics: When doxycycline-induced overexpresses 8 liver-enriched transcription factors (LETFs) (from parent cell line).
CC   Characteristics: The parent cell line was modified to enable switching between proliferation and expression of proteins for liver function in response to heat by introducing a new heat-inducible gene expression/amplification system (pHSP-tTA-TRE-tTA-pEF1a-Puro) which was transferred using a PiggyBac transposon vector (PubMed=34149171).
CC   Transfected with: MGI; MGI:99480; Cebpa.
CC   Transfected with: MGI; MGI:88373; Cebpb.
CC   Transfected with: MGI; MGI:104982; Cebpg.
CC   Transfected with: MGI; MGI:1347476; Foxa2.
CC   Transfected with: MGI; MGI:98504; Hnf1a.
CC   Transfected with: MGI; MGI:98505; Hnf1b.
CC   Transfected with: MGI; MGI:109128; Hnf4a.
CC   Transfected with: MGI; MGI:1196423; Onecut1.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Omics: Transcriptome analysis by microarray.
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C38756; Hepatocellular carcinoma of the mouse
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_RI52 ! Hepa/8F5
SX   Female
CA   Cancer cell line
DT   Created: 17-03-22; Last updated: 29-06-23; Version: 4
//
RX   PubMed=34149171; DOI=10.1007/s10616-021-00457-4;
RA   Kitano H., Nagae Y., Kawabe Y., Ito A., Kamihira M.;
RT   "Development of a genetically modified hepatoma cell line with
RT   heat-inducible high liver function.";
RL   Cytotechnology 73:353-362(2021).
//