ID   ART [Human OVCAR-8 contaminated cell line]
AC   CVCL_B0CC
DR   ATCC; CRL-3468
DR   cancercelllines; CVCL_B0CC
DR   Wikidata; Q108819855
RX   PubMed=23843634;
RX   PubMed=29091757;
CC   Problematic cell line: Contaminated. Parent cell line (NCI-ADR-RES) has been shown to be a OVCAR-8 derivative.
CC   Population: Caucasian.
CC   Characteristics: Engineered to overexpress the SV40 large T antigen.
CC   Virology: Can be used to evaluate the infectious entry of polyomaviruses (BKPyV, JCPyV, SV40, MCPyV) and human papillomaviruses. Pathogenic variants of the JCPyV virus previously believed to be non-infectious can infect this cell line (ATCC=CRL-3468).
CC   Doubling time: ~34 hours (ATCC=CRL-3468).
CC   Sequence variation: Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Gln26Arg (c.77A>G); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:3430; ERBB2; Simple; p.Gly776Val (c.2327G>T); ClinVar=VCV000163410; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Pro121His (c.362C>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; c.376-1G>A (p.Tyr126_Lys132del, c.376_396del21); ClinVar=VCV000481003; Zygosity=Homozygous; Note=Splice acceptor mutation (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
ST   Source(s): ATCC=CRL-3468
ST   Amelogenin: X
ST   CSF1PO: 11
ST   D13S317: 12
ST   D16S539: 13
ST   D5S818: 12
ST   D7S820: 20
ST   TH01: 7
ST   TPOX: 8
ST   vWA: 16,17
DI   NCIt; C105555; High grade ovarian serous adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1629 ! OVCAR-8
SX   Female
AG   64Y
CA   Cancer cell line
DT   Created: 23-09-21; Last updated: 14-08-25; Version: 12
//
RX   PubMed=23843634; DOI=10.1128/JVI.01189-13; PMCID=PMC3754014;
RA   Pastrana, Diana V.
RA   Ray, Upasana
RA   Magaldi, Thomas G.
RA   Schowalter, Rachel M.
RA   Cuburu, Nicolas
RA   Buck, Christopher B.
RT   "BK polyomavirus genotypes represent distinct serotypes with distinct
RT   entry tropism.";
RL   J. Virol. 87:10105-10113(2013).
//
RX   PubMed=29091757; DOI=10.1016/j.celrep.2017.10.027; PMCID=PMC5687836;
RA   Geoghegan, Eileen M.
RA   Pastrana, Diana V.
RA   Schowalter, Rachel M.
RA   Ray, Upasana
RA   Gao, Wei
RA   Ho, Mitchell
RA   Pauly, Gary T.
RA   Sigano, Dina M.
RA   Kaynor, Campbell
RA   Cahir-McFarland, Ellen
RA   Combaluzier, Benoit
RA   Grimm, Jan
RA   Buck, Christopher B.
RT   "Infectious entry and neutralization of pathogenic JC
RT   polyomaviruses.";
RL   Cell Rep. 21:1169-1179(2017).
//