ID   U2OS DR-GFP
AC   CVCL_B0A7
SY   U-2 OS DRGFP
DR   ATCC; CRL-3455
DR   Wikidata; Q108821411
CC   Population: Caucasian.
CC   Characteristics: Transfected with a DR-GFP reporter construct. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP.
CC   Characteristics: Can be used to monitor homologous recombination efficiency using the DR-GFP reporter (ATCC=CRL-3455).
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
ST   Source(s): ATCC
ST   Amelogenin: X
ST   CSF1PO: 13
ST   D13S317: 13
ST   D16S539: 11,12
ST   D5S818: 11
ST   D7S820: 11,12
ST   TH01: 6,9.3
ST   TPOX: 11,12
ST   vWA: 14,18
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 23-09-21; Last updated: 05-10-23; Version: 5
//