ID   NRK-4xlambdaN22-3xmEGFP-M9
AC   CVCL_AV97
SY   NRK 4xlN22-3xmEGFP-M9
DR   CLS; 500672
DR   Wikidata; Q54931029
RX   PubMed=17603490;
CC   Characteristics: Transfected with a construct containing four copies to the 22-amino-acid peptide ligand from phage lambda N protein (lambda-N22), spaced by a 21 amino acid linker (PPLDGAGAGAGAGAGAGGLAT) and fused to 3 monomeric meGFP and a M9 nuclear localization signal. This construct allows binding to a 15-nt RNA hairpin termed boxB that can be fused to any RNA molecule allowing specific RNA visualization in live cells (PubMed=17603490).
CC   Genetic integration: Method=Transfection; Gene=FPbase; QKFJN; mEGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03045; Escherichia phage lambda N (Note=p.Met1_Asn22 = lambdaN22).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Breed/subspecies: Osborne-Mendel; RS=RS_0000629.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_3758 ! NRK
CA   Spontaneously immortalized cell line
DT   Created: 02-05-16; Last updated: 10-04-25; Version: 11
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RX   PubMed=17603490; DOI=10.1038/nmeth1065;
RA   Daigle N., Ellenberg J.;
RT   "LambdaN-GFP: an RNA reporter system for live-cell imaging.";
RL   Nat. Methods 4:633-636(2007).
//