ID   WISH-Mx2/EGFP
AC   CVCL_A8RR
SY   WISH-Mx2/eGFP; WISH-Mx/eGFP; L1G3
DR   Wikidata; Q108821517
RX   PubMed=22549076;
RX   PubMed=27346232;
RX   PubMed=31699391;
CC   Problematic cell line: Contaminated. Parent cell line (WISH) has been shown to be a HeLa derivative.
CC   Population: African American.
CC   Biotechnology: Used for a reporter gene assay (RGA) of the human type 1 interferon potency (PubMed=22549076).
CC   Characteristics: Expression of eGFP is under the control of the control of the mouse Mx2 promoter (PubMed=22549076).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1909 ! WISH
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 23-09-21; Last updated: 10-04-25; Version: 7
//
RX   PubMed=22549076; DOI=10.1016/j.jim.2012.04.010;
RA   Burgi M., Prieto C., Etcheverrigaray M., Kratje R., Oggero M.,
RA   Bollati-Fogolin M.;
RT   "WISH cell line: from the antiviral system to a novel reporter gene
RT   assay to test the potency of human IFN-alpha and IFN-beta.";
RL   J. Immunol. Methods 381:70-74(2012).
//
RX   PubMed=27346232; DOI=10.1016/j.jbiotec.2016.06.021;
RA   Burgi M., Zapol'skii V.A., Hinkelmann B., Koster M., Kaufmann D.E.,
RA   Sasse F., Hauser H., Etcheverrigaray M., Kratje R.,
RA   Bollati-Fogolin M., Oggero M.;
RT   "Screening and characterization of molecules that modulate the
RT   biological activity of IFNs-I.";
RL   J. Biotechnol. 233:6-16(2016).
//
RX   PubMed=31699391; DOI=10.1016/j.bioorg.2019.103372;
RA   Burgi M., Hernandez P., Cabrera M., Cerecetto H., Gonzalez M.,
RA   Kratje R., Raimondi A., Oggero M., Bollati-Fogolin M.;
RT   "Identification and characterization of human interferon alpha
RT   inhibitors through a WISH cell line-based reporter gene assay.";
RL   Bioorg. Chem. 94:103372.1-103372.10(2020).
//