ID   HT29-5M21
AC   CVCL_A8Q0
DR   cancercelllines; CVCL_A8Q0
DR   ECACC; 15121706
DR   Wikidata; Q102114164
RX   PubMed=9579576;
RX   PubMed=15050369;
CC   Population: Caucasian.
CC   Characteristics: Mucus-secreting clone of HT-29 which forms a monolayer of polarized cells with strong anti-gastric mucin immunoreactivity and mainly expresses MUC5AC and MUC5B. Resistant to methotrexate and sensitive to 5-fluorouracil.
CC   Selected for resistance to: ChEBI; CHEBI:44185; Methotrexate (MTX).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
ST   Source(s): ECACC
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 11
ST   D16S539: 11,12
ST   D18S51: 13
ST   D21S11: 29
ST   D3S1358: 15
ST   D5S818: 11,12
ST   D7S820: 10
ST   D8S1179: 10
ST   FGA: 20,22
ST   Penta D: 11
ST   Penta E: 14,16
ST   TH01: 6,9
ST   TPOX: 8,9
ST   vWA: 17
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0320 ! HT-29
SX   Female
AG   44Y
CA   Cancer cell line
DT   Created: 29-10-20; Last updated: 05-10-23; Version: 8
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RX   PubMed=9579576; DOI=10.1002/(SICI)1097-0215(19980504)76:3<383::AID-IJC16>3.0.CO;2-C;
RA   Lesuffleur T., Violette S., Vasile-Pandrea I., Dussaulx E., Barbat A.,
RA   Muleris M., Zweibaum A.;
RT   "Resistance to high concentrations of methotrexate and 5-fluorouracil
RT   of differentiated HT-29 colon-cancer cells is restricted to cells of
RT   enterocytic phenotype.";
RL   Int. J. Cancer 76:383-392(1998).
//
RX   PubMed=15050369; DOI=10.1016/j.biolcel.2003.12.005;
RA   Leteurtre E., Gouyer V., Rousseau K., Moreau O., Barbat A.,
RA   Swallow D.M., Huet G., Lesuffleur T.;
RT   "Differential mucin expression in colon carcinoma HT-29 clones with
RT   variable resistance to 5-fluorouracil and methotrexate.";
RL   Biol. Cell 96:145-151(2004).
//